Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance

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Red Sea Atlantis II brine pool nitrilase with unique thermostability profile and heavy metal tolerance

BACKGROUND Nitrilases, which hydrolyze nitriles in a one-step reaction into carboxylic acids and ammonia, gained increasing attention because of the abundance of nitrile compounds in nature and their use in fine chemicals and pharmaceutics. Extreme environments are potential habitats for the isolation and characterization of extremozymes including nitrilases with unique resistant properties. Th...

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Isolation and characterization of a heavy metal-resistant, thermophilic esterase from a Red Sea brine pool.

The Red Sea Atlantis II brine pool is an extreme environment that displays multiple harsh conditions such as high temperature, high salinity and high concentrations of multiple, toxic heavy metals. The survival of microbes in such an environment by utilizing resistant enzymes makes them an excellent source of extremophilic enzymes. We constructed a fosmid metagenomic library using DNA isolated ...

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Unique Prokaryotic Consortia in Geochemically Distinct Sediments from Red Sea Atlantis II and Discovery Deep Brine Pools

The seafloor is a unique environment, which allows insights into how geochemical processes affect the diversity of biological life. Among its diverse ecosystems are deep-sea brine pools - water bodies characterized by a unique combination of extreme conditions. The 'polyextremophiles' that constitute the microbial assemblage of these deep hot brines have not been comprehensively studied. We rep...

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Viruses-to-mobile genetic elements skew in the deep Atlantis II brine pool sediments

The central rift of the Red Sea has 25 brine pools with different physical and geochemical characteristics. Atlantis II (ATIID), Discovery Deeps (DD) and Chain Deep (CD) are characterized by high salinity, temperature and metal content. Several studies reported microbial communities in these brine pools, but few studies addressed the brine pool sediments. Therefore, sediment cores were collecte...

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ژورنال

عنوان ژورنال: BMC Biotechnology

سال: 2016

ISSN: 1472-6750

DOI: 10.1186/s12896-016-0244-2